Confocal microscopy core facility
The confocal microscopy core facility provides "open access" confocal laser scanning microscopy (LSM) technology to all Tipp-Täpp/UT investigators. Though we are not a service facility, thus we do not routinely perform experiments for researches, but we will provide access to imaging platforms and assistance on the imaging aspects of the user's experiment.
The Facility's equipment includes a:
- Zeiss LSM 510 META Confocal Microscope
- Zeiss LSM 780/ELYRA (LSM 780 with super-resolution SIM) Confocal Microscope
- Zeiss Axio Observe Z1 Inverted Microscope with Neurolucida/Stereo Investigator system.
Imaging of both fixed specimens and live cells is provided. For live cell imaging, cells are placed in a temperature and CO2 controlled chamber or an open chamber allowing acquisition over several minutes to days. Inverted brightfield and fluorescent microscope could be used to acquire accurate stereological data or automatic neuron tracing and reconstruction. Fluorescent recovery after photo-bleaching (FRAP), fluorescence resonance energy transfer (FRET), and co-localization studies can be conducted as well as imaging of UV excitable dyes.
Several software packages are available to users including:
- Zeiss ZEN Lite software
- Zeiss LSM Image Browser
- Stereo Investigator software
- Neurolucida software
- Free downloads: ImageJ and FIJI